| 摘要: |
| 目的:观察早期肾透明细胞癌及癌旁0.5、1.0、2.0 cm组织上皮膜抗原（epithelial membrane antigen,EMA）、肾细胞癌标志物（renal cell carcinoma marker,RCC-Ma）的表达差异，初步探讨早期肾癌行肾部分切除术时的安全切除边距。方法:构建包含44例早期肾透明细胞癌及癌旁0.5、1.0、2.0 cm肾皮质的组织芯片，采用免疫组化染色分别观察癌及癌旁（距肿瘤边缘0.5、1.0、2.0 cm）组织EMA、RCC-Ma的表达差异。结果:EMA、RCC-Ma在癌及癌旁组织均为细胞膜和（或）胞质染色。癌组织EMA和RCC-Ma强阳性表达率明显高于癌旁组织各组（15.91%/18.18% vs 84.09%/79.55%、86.36%/77.27%、79.55%/75.00%，P<0.01）；而癌旁组织各组（距肿瘤边缘0.5、1.0、2.0 cm）间差异无统计学意义（P>0.05）。结论:0.5 cm可能是早期肾透明细胞癌腹腔镜肾部分切除术安全的切除边距。 |
| 关键词: 上皮膜抗原 肾细胞癌标志物 肾肿瘤 组织芯片 肾切除术 |
| DOI：10.3724/SP.J.1008.2009.0672 |
| 投稿时间：2008-11-21修订日期：2009-01-04 |
| 基金项目:上海市科委医学引导科技项目（074119618）. |
|
| Determination of safe margin of laparoscopic partial nephrectomy for early renal carcinoma |
| SHEN Yi-zhen1，ZHENG Jun-hua2*，YU Guan-zhen3，GAO Yi1，MIN Zhi-lian1 |
| (1.Department of Urology，Changzheng Hospital，Second Military Medical University，Shanghai 200003，China;2.Department of Urology，The 10th People’s Hospital of Shanghai，Tongji University，Shanghai 200072;3.Department of Oncology，Changzheng Hospital，Second Military Medical University，Shanghai 200003) |
| Abstract: |
| Objective:To observe the differential expression of epithelial membrane antigen (EMA) and renal cell carcinoma marker (RCC-Ma) in early renal carcinoma and corresponding adjacent (0.5, 1.0, and 2.0 cm) tissues, so as to estimate the safe margin of laparoscopic partial nephrectomy for renal tumors.Methods:Tissue microarrys, including 44 tissues of clear cell renal cell carcinoma［A］ and normal renal cortex tissues of different distances to the tumor：0.5 cm［B］，1.0 cm［C］ and 2.0 cm［D］, were constructed.The expression of EMA and RCC-Ma was examined by immunohistochemical staining in the four groups. Results:Intensive membranous and(or) cytoplasmic staining of EMA and RCC-Ma was observed in the cancer and adjacent tissues. The positive rate in group A was significantly higher than those of the other three groups（A \[15.91%/18.18%\]，B\[84.09%/79.55%\]，C\[86.36%/77.27%\] and D\[79.55%/75.00%\], P<0.01）.The positive rates of EMA and RCC-Ma expression were not significantly different between the group B, C, and D（P>0.05）.Conclusion:Our data denote that, when laparoscopic partial nephrectomy is done for early renal carcinoma, at least 5 mm of normal parenchyma tissue beyond the pesudocapsule should be excised with the tumor. |
| Key words: epithelial membrane antigen renal cell carcinoma marker kidney neoplasms tissue microarray nephrectomy |
