| 摘要: |
| 目的:探讨人促红细胞生成素(EPO)对腹部开放伤加人工海水浸泡所致急性肾损伤大鼠的保护作用。方法:健康、雄性Wistar大鼠随机分成EPO预处理组、观察组、小剂量EPO救治组、大剂量EPO救治组,每组15只动物。腹部开放伤加人工海水(22℃)浸泡，制备急性肾损伤动物模型,检测各组实验动物血肌酐、尿素氮、肌酸激酶、肌酸激酶同工酶、TNF-α、IL-6、补体C3a、C反应蛋白、肾组织匀浆超氧化物歧化酶(SOD)水平，并观察肾脏病理学变化。结果:各组大鼠在腹部开放伤加海水浸泡3 h后均发生了急性肾损伤改变,血清肌酐、尿素氮升高,但仍可存活;EPO预处理组与观察组及小/大剂量EPO救治组比较,大鼠血肌酐、尿素氮、肌酸激酶、肌酸激酶同工酶显著降低，TNF-α、IL-6、C3a、C反应蛋白等炎性因子水平明显降低，肾组织匀浆SOD明显升高，肾脏近曲小管坏死程度计分明显下降。而大、小剂量救治组间各项指标比较则无统计学差异（P>0.05）。结论: EPO预处理对腹部开放伤加海水浸泡所致急性肾损伤大鼠有保护作用。 |
| 关键词: 促红细胞生成素 腹部损伤 海水浸泡 急性肾损伤 |
| DOI：10.3724/SP.J.1008.2009.0527 |
| 投稿时间：2008-12-04修订日期：2009-02-23 |
| 基金项目:海军后勤部卫生部医药卫生基金(04-3304). |
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| Protective effect of human erythropoietin on acute renal injury caused by abdomen open injury plus artificial seawater immersion in rats |
| ZHANG Zhi-yong1,2,ZHOU Chun-hua2,LEI Xia2,YUAN Fa-huan1* |
| (1.Department of Nephrology,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China;2.Department of Nephrology,Naval General Hospital of PLA,Beijing 100037) |
| Abstract: |
| Objective: To investigate the protective effect of erythropoietin (EPO) on the acute renal injuries caused by abdomen open injury plus seawater immersion in rats.Methods: Sixty healthy male Wistar rats (clean grade) were evenly randomized into four groups,namely,EPO pre-treatment group,observation group,low-dose EPO treatment group and high-dose EPO treatment group.Acute renal injury was induced by abdomen open injury plus artificial seawater immersion (22℃).The serum creatine,BUN,creatine kinase,creatine kinase isoenzyme,TNF-α,IL-6,complement C3a,C-reactive protein,renal homogenate superoxide dismutase (SOD),and the renal pathological changes were observed and compared between different groups.Results: Acute renal injury was observed in all groups 3 hours after abdomen open injury plus seawater immersion,with increased serum creatine and BUN,but the rats survived after treatment.The serum creatine,BUN,creatine kinase,and creatine kinase isoenzyme in EPO pre-treatment group were significantly lower than those of the other 3 groups; the levels of TNF-α,IL-6,complement C3a,and C-reactive protein were also obviously decreased; the renal homogenate SOD was obviously increased; and the score of renal proximal tubule necrosis was obviously decreased.However,no significant differences were found between the high- and low-dose EPO groups concerning all the parameters (P>0.05).Conclusion: EPO pre-treatment has a protective effect on the acute renal injury induced by abdomen open injury plus seawater immersion in rats. |
| Key words: erythropoietin abdominal injuries seawater immersion acute kidney injuries |
