| 摘要: |
| 目的探讨复方血栓通胶囊对糖尿病大鼠肾脏的保护作用。方法雄性SD大鼠予链脲佐菌素(STZ)60 mg/kg单次腹腔注射诱发糖尿病,3 d后尾静脉血糖高于16.7 mmol/L者视为造模成功。实验动物分为正常对照组(NG)、糖尿病组(DM)、糖尿病复方血栓通胶囊处理组(XST)、糖尿病α-硫辛酸处理组(ALA),每组均10只。12周后比较各组体质量、血糖、肾脏/体质量、血脂、24 h尿蛋白含量、血肌酐、尿素氮。透射电镜及光镜观察肾脏超微结构改变。结果与正常对照组相比,DM组大鼠24 h尿蛋白排泄量明显增加(P<0.05),经复方血栓通及α-硫辛酸处理后,24 h尿白蛋白排泄量明显降低(P<0.05)；与正常对照组相比,各模型组大鼠左肾质量/体质量比明显升高,差异有统计学意义(P<0.01),经复方血栓通胶囊、α-硫辛酸处理后大鼠左肾质量/体质量比降低,其中XST组与DM组相比差异有统计学意义(P<0.05)。电镜下可见DM组大鼠肾小球基底膜厚度不均匀增厚,足突融合、消失,系膜细胞增生；经复方血栓通胶囊、α-硫辛酸处理后,上述病变有所好转。结论复方血栓通胶囊可降低24 h尿白蛋白排泄量,降低肾脏肥大系数(左肾质量/体质量),改善肾小球基底膜结构,对糖尿病大鼠肾脏具有保护作用。 |
| 关键词: 糖尿病肾病 氧化应激 中药复方 超微结构 硫辛酸 |
| DOI：10.3724/SP.J.1008.2010.01091 |
| 投稿时间：2010-05-27修订日期：2010-09-02 |
| 基金项目: |
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| Protective effect of compound Xueshuantong capsule against damage of diabetic nephropathy in rat diabetic model |
| LIU Zhi-min*,XING Yu-wei, TANG Wen-jia, ZOU Jun-jie, SHI Yong-quan, LIU Zhi-min* |
| (Department of Endocrinology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China) |
| Abstract: |
| ObjectiveTo investigate the protective effect of compound Xueshuantong capsule against damage of diabetic nephropathy in rat diabetic model. MethodsMale SD rats were given intraperitoneal injection of streptozotocin (STZ) at 60 mg/kg, and diabetic rat model was deemed successfully induced when the blood glucose level ≥16.7 mmol/L 3 days after injection. Thirty model rats were randomly divided into the following 3 groups equally: diabetic model group (DM), compound Xueshuantong capsule group (XST), and α-lipoic acid treatment group (ALA). Another 10 normal rats served as normal control group (NG). The body weight, blood glucose, blood fat, kidney weight/body weight(KW/BW), 24 h-urinary protein excretion, urea nitrogen, and serum creatinine were observed and compared between different groups after 12 weeks’ treatment. The ultrastructure changes of kidney were observed by electron microscopy and light microscope. ResultsCompared with NG group, rats in DM group had a higher 24 h-urinary protein excretion (P<0.05), and those in XST group and ALA group had a significantly lower one compared with the DM group (P<0.05). Compared with NG group, rats in the other 3 groups had a higher KW/BW, with significant difference found between DM group and XST group(P<0.05). Electron microscopic results showed uneven glomerular basement membrane thickening, foot process integration, and mesangial cell proliferation in the model group; all the above changes were improved in the XST and ALA groups. ConclusionCompound Xueshuantong capsule can lower the 24 h-urinary protein secretion, decrease KW/BW, and improve the structure of glomerular basement membrane, thus exerting renal protective effect in diabetic rats. |
| Key words: diabetic nephropathy oxidative stress traditional Chinese complex medicine ultrastructure thioctic acid |
