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| 物样品电镜超薄切片染色技术的改进 |
| 唐〓凯1,2△,叶煦亭2△,范晓燕2,晋若冰2,林方兴2,金〓婵2,汤〓莹2,马春旺1,杨勇骥2* |
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| (1. 河南师范大学物理与信息工程学院物理学系, 新乡 453007;2. 第二军医大学基础部生物物理研究所, 上海 200433) |
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| 摘要: |
| [摘要\] 目的 提高透射电镜生物样品超薄切片的染色效率和染色质量。方法 在传统染色方法基础上进行改进, 采用“插入式滴染法”和连续染色方式。 结果 在较短时间内完成大批生物样品超薄切片的电子染色。 结论 与传统方法相比省时省药, 减少污染概率, 电镜观察结构清晰, 反差较好。 |
| 关键词: 透射电子显微镜检查 生物样品制备 超薄切片染色 |
| DOI：10.3724/SP.J.1008.2011.0559 |
| 投稿时间：2011-01-14修订日期：2011-04-20 |
| 基金项目: |
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| Improvement in specimen preparation of ultra thin section staining for transmission electron microscopy |
| 唐〓凯1,2△, 叶煦亭2△, 范晓燕2, 晋若冰2, 林方兴2, 金〓婵2, 汤〓莹2, 马春旺1, 杨勇骥2* |
| (Institute of Biophysics, Second military medical university) |
| Abstract: |
| \[Abstract\]〓Objective〓 To improve the staining efficiency and quality of biological specimens ultrathin section for transmission electron microscopy. Methods〓 The “plugin staining method” and continuous staining were developed by modifying the traditional staining method. Results〓 Using our method, we completed the electron staining of a large number of ultrathin sections of biological specimens of within a short period of time. 〖WTHZ〗Conclusion〓 Compared with traditional method, the new method can save time and drug, and reduce the possibility of staining contamination. The ultrastructure observed by electron microscope is more clear and distinct. |
| Key words: transmission electron microscopy specimen preparation ultra-thin section’s staining |
