| 摘要: |
| 目的观察川芎嗪对转化生长因子β1(TGF-β1)诱导的人肾小球系膜细胞增殖及Ⅳ型胶原分泌的影响。方法采用TGF-β1诱导肾小球系膜细胞增殖和Ⅳ型胶原表达以模拟肾脏疾病中的细胞纤维化,实验分为5组:空白组,对照组,川芎嗪高、中、低剂量(终浓度分别为100、30、10 μg/ml)组。MTT法检测肾小球系膜细胞的增殖抑制率, ELISA法检测肾小球系膜细胞Ⅳ型胶原的表达。结果川芎嗪高剂量(100 μg/ml)能够抑制TGF-β1诱导的肾小球系膜细胞增殖(P<0.01);川芎嗪可抑制TGF-β1诱导的肾小球系膜细胞中Ⅳ型胶原的表达,以高剂量组(100 μg/ml)效果最为显著(P<0.01)。结论川芎嗪可抑制TGF-β1诱导的肾小球系膜细胞增殖及Ⅳ型胶原分泌。 |
| 关键词: 川芎嗪 肾小球系膜细胞 转化生长因子β1 胶原Ⅳ型 |
| DOI：10.3724/SP.J.1008.2011.0633 |
| 投稿时间：2011-03-01修订日期：2011-05-05 |
| 基金项目: |
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| Effect of ligustrazine hydrochloride injection on TGF-β1-induced proliferation and type Ⅳ collagen secretion in human mesangial cells |
| XUE Xiao-chun1*,LIU Fang2 |
| (1. Department of Pharmacy, No.101 Hospital of PLA, Wuxi 214044, Jiangsu, China;2. Department of Pharmacy, No.455 Hospital of PLA, Shanghai 200052, China) |
| Abstract: |
| ObjectiveTo observe the effect of ligustrazine hydrochloride(LHC) injection on TGF-β1-induced proliferation and type Ⅳ collagen secretion in the human mesangial cells(HMCs).MethodsThe interstitial fibrosis in kidney disease was mimicked by inducing proliferation and type Ⅳ collagen secretion in HMCs with TGF-β1. The experiment was divided into 5 groups: blank, control, low, medium, and high(10, 30, and 100 μg/ml)ligustrazine hydrochloride groups. MTT method was adopted to examine the proliferation and inhibition rate of HMCs. Enzyme-linked immunosorbent assay was used to determine the production of type Ⅳ collagen in cultured HMCs.ResultsLHC at high concentration (100 μg/ml) significantly inhibited the proliferation of HMCs (P<0.01). LHC also inhibited the production of type Ⅳ collagen, with the significant inhibition found when at the concentration of 100 μg/ml (P<0.01).ConclusionLHC can greatly inhibit TGF-β1-induced proliferation and expression of type Ⅳ collagen in human mesangial cells. |
| Key words: ligustrazine hydrochloride mesangial cells transforming growth factor-β1 |
