| 摘要: |
| 目的 探讨内皮素-1(ET-1)、ET-2、ET-3、内皮素受体A(ETRA)、ETRB和内皮素转换酶(ECE)在8周糖尿病SD大鼠视网膜中的表达。 方法 采用限制性片段差异显示PCR(RFDD-PCR)技术建立正常和8周糖尿病大鼠视网膜基因差异表达谱,并以半定量RT-PCR和蛋白免疫印迹方法分别对两组间表达存在差异的6个基因(ET-1、ET-2、ET-3、ETRA、ETRB、ECE)的表达进行验证。 结果 RFDD-PCR结果显示,糖尿病组ET-1、ET-2、ET-3、ETRA、ETRB和ECE表达上调。半定量RT-PCR结果和蛋白免疫印迹结果显示,6种基因和蛋白在糖尿病组的表达(相对光密度比值)高于正常组,表达差异具有统计学意义(P均<0.05,P均<0.01)。结论 ET-1、ET-2、ET-3、ETRA、ETRB和ECE在糖尿病视网膜中呈高表达,提示这6种基因可能参与了糖尿病视网膜的发病过程。 |
| 关键词: 糖尿病视网膜病变 内皮素 内皮素受体 内皮素转换酶 |
| DOI：10.3724/SP.J.1008.2012.00436 |
| 投稿时间：2011-10-08修订日期：2011-12-29 |
| 基金项目:上海市卫生局青年课题 (2010Y030). |
|
| Expression of endothelins and endothelin receptors in retina of diabetic rats |
| YUAN Ai-hua1,2,JIANG Ping1,MEI Yan2,YANG Hui-jun2* |
(1. Department of Genetics, Shanghai Mental Health Center, Shanghai Jiaotong University School of Medicine, Shanghai200030, China
2. Department of Human Anatomy and Embryology, West China Medical Centre, Sichuan University, Chengdu 610041, Sichuan, China
*Corresponding author.) |
| Abstract: |
| Objective To investigate the expression of endothelin-1 (ET-1), ET-2, ET-3,endothelin receptor A (ETRA), ETRB and endothelin coverting enzyme (ECE) in the retinas of 8-week-old diabetic SD rats. Methods The retinal gene expression profiles of healthy and 8-week-old diabetic rats were constructed with restriction fragment differential display polymerase chained reaction (RFDD-PCR), and the differential expression of ET-1, ET-2, ET-3, ETRA, ETRB and ECE was verified using semi-quantitative RT-PCR and Western blotting analysis.Results The Results of RFDD-PCR showed that the expression of ET-1, ET-2, ET-3, ETRA, ETRB and ECE was up-regulated in diabetic retina. The Results of semi-quantitative RT-PCR and Western blotting analysis showed that the expression levels of the six genes and proteins (relative D ratio) in diabetic group were significantly higher than those in the normal retinas (P<0.05 and P<0.01, respectively). Conclusion The expression of ET-1, ET-2, ET-3, ETRA, ETRB and ECE is up-regulated in diabetic retina, suggesting that the six genes may be involved in the pathgenesis of diabetic retina. |
| Key words: diabetic retinopathy endothelin endothelin receptor endothelin converting enzyme |
