| 摘要: |
| 氨基酸和小肽是微生物内重要的活性组分和代谢产物。随着现代分析技术的日益革新,微生物氨基酸和小肽的定性定量分析方法 也在不断发展。本文首先从淬灭、提取、样品衍生化和浓缩几个方面对微生物样品前处理方法 做了介绍；进而分别从分离技术和检测方法 两方面综述了氨基酸和小肽研究中所采用相关技术的特点及应用实例,并系统分析了各种方法 的优缺点,同时展望了其发展趋势。 |
| 关键词: 氨基酸类 肽类 微生物学技术 |
| DOI：10.3724/SP.J.1008.2012.0216 |
| 投稿时间：2011-10-08修订日期：2011-11-22 |
| 基金项目: |
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| Analysis methods of amino acids and small peptides from microorganism: recent advance |
| WU Hai-tang,CHEN Xiao-fei,WANG Hui,CAO Ying-ying,ZHU Zhen-yu* |
(Department of Drug Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
*Corresponding author.) |
| Abstract: |
| Amino acids and small peptides are important active constituents and metabolites of microorganism. With the progression in modern analytical techniques, the qualitative and quantitative analysis methods of amino acids and small peptides from microorganism also keep improving. In this paper, the pretreatment methods were discussed from the quenching, extracting, derivatization and concentration steps. Furthermore, the properties and practical application of the commonly used separation and detection methods were discussed. Meanwhile, the prospects of the analysis methods for amino acids and small peptides from microorganism were also highlighted. |
| Key words: amino acid small peptide microorganism analytical techniques review |
