| 摘要: |
| 目的 探讨稳定微管能否减少心肌缺血再灌注损伤。方法 (1)将离体大鼠心脏分为对照组、缺血组、缺血+0.1 μmol/L泰素和缺血+1 μmol/L泰素组(n=15)。每组先给予15 min平衡后，对照组继续给予50 min常氧灌注；缺血组给予20 min常氧灌注+30 min缺血处理；缺血+0.1 μmol/L(或1 μmol/L)泰素组给予20 min常氧灌注，30 min缺血处理，整个过程中均给予0.1 μmol/L(或1 μmol/L)泰素灌流。比较各组心律失常情况，观察微管形态结构并进行断裂评分。(2)将离体大鼠心脏分为正常对照组、缺血再灌注组和1 μmol/L泰素组(n=8)。缺血再灌注组进行Langendorff灌流，结扎左前降支30 min，再灌注120 min；1 μmol/L泰素组给予1 μmol/L进行灌流，其余处理同缺血再灌注组。灌流结束即刻冠脉内灌入伊文思蓝进行染色，比较各组心肌梗死面积。结果 (1)0.1和1 μmol/L泰素均能有效降低缺血性室性心律失常的发生，降低室性心律失常评分，且呈剂量依赖性(P<0.05)；并均能降低室性心动过速的发生率(P<0.05)。(2)0.1 μmol/L泰素灌流能降低微管断裂评分。(3)1 μmol/L泰素灌流能明显缩小缺血再灌注心脏梗死区面积(P<0.05)。结论 稳定微管能减少心肌缺血再灌注损伤。 |
| 关键词: 泰素 微管 缺血性室性心律失常 心肌梗死 再灌注损伤 |
| DOI：10.3724/SP.J.1008.2013.00167 |
| 投稿时间：2012-10-25修订日期：2012-12-31 |
| 基金项目:无锡市科技局医学技术重大项目(YGZ1104). |
|
| Experimental research on stabilizing microtubules to decrease myocardial injury |
| FENG Jian,WU Ding-ye,YOU Hua-yan,LI Jian,WANG Qiang,CAO Hua-ming* |
(Department of Cardiology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi 213023, Jiangsu, China
*Corresponding author.) |
| Abstract: |
| Objective To study whether stabilizing microtubules can decrease myocardial ischaemic-reperfusion injury. Methods (1) The isolated rat hearts were divided into four groups (n=15): control group, ischemic group, ischemic+0.1 μmol/L Taxol group, and ischemic+1 μmol/L Taxol group. All the groups were given a 15-min equilibration and then followed by different treatments: control group, 50 min normoxic superfusion; ischemia group, 20 min normoxic superfusion plus 30 min ischemia; and Taxol groups, 20 min normoxic superfusion plus 30 min ischemia, plus 0.1 or 1 μmol/L Taxol throughout 50-min superfusion period. Arrthymias scores were assessed and compared between different groups; the structure of the microtubules was observed and its breakage score was obtained. (2) The isolated rat hearts were divided into 3 groups (n=8): normal control group, ischemic/reperfusion (I/R) group, and 1 μmol/L Taxol group. The I/R group was Langendorff-perfused; the left anterior descending branch was ligated for 30 min and reperfused for 120 min. The Taxol group received 1 μmol/L Taxol and other treatments were similar to the I/R group. Evans blue perfusion was used to observe the infarct size of each group. Results Stabilizing microtubules with Taxol (0.1 μmol/L or 1 μmol/L) reduced ischaemic ventricular arrhythmia in a dose-dependent fashion (P<0.05); it also significantly reduced arrhythmia scores and the incidence of ventricular tachycardia (P<0.05). Taxol at 0.1 μmol/L greatly decreased microtubule breakage score, and at 1 μmol/L significantly reduced the infarct size compared with the control group (P<0.05). Conclusion Stabilizing microtubules can reduce myocardial ischaemic-reperfusion injury. |
| Key words: Taxol microtubules ischaemic ventricular arrhythmia myocardial infarction reperfusion injury |
