| 摘要: |
| 目的 研究再生基因蛋白IV (regenerating gene typeIV, RegIV)及磷脂酰肌醇3-激酶(phosphatidylinositol-3-kinase，PI3K)、Akt蛋白在胃腺癌中的表达及与其临床意义。方法 应用S-P免疫组化技术检测63例胃腺癌及其相应的癌旁正常组织中RegIV和PI3K、Akt蛋白的表达。结果 63例胃腺癌组织中RegIV、PI3K、Akt蛋白的阳性表达率50.7% (32/63)、68.3% (43/63) 、60.3% (38/63)均高于癌旁正常组织19.0% (12/63)、20.6% (13/63)、9.5% (6/63)，差异具统计学意义（P<0.05）；RegIV的表达与肿瘤分化程度相关，有统计学意义（P<0.05）;PI3K蛋白的表达与肿瘤分化程度、浸润深度、淋巴结转移、临床分期相关有统计学意义（P<0.05）；Akt蛋白的表达与淋巴结转移相关有统计学意义（P<0.05）。RegⅣ与PI3K、Akt蛋白在胃腺癌组织中的表达呈正相关关系(rs=0.284、305，P<0.05)， PI3K与Akt蛋白在胃腺癌组织中的表达呈正相关关系( rs=0.423，P<0.05)。结论 RegIV可能通过激活PI3K/Akt信号系统在肿瘤的发生发展中发挥重要作用。 |
| 关键词: 再生基因蛋白IV 胃腺癌 磷脂酰肌醇3-激酶 Akt 免疫组织化学 |
| DOI：10.3724/SP.J.1008.2014.00786 |
| 投稿时间：2013-11-05修订日期：2014-05-19 |
| 基金项目:开封市科技攻关计划项目 |
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| Expressions and clinical significance of RegIV and PI3K/Akt signaling proteins in human gastric adenocarcinoma |
| yangdesheng,kangyuhua,lifuchun,sunzhulei,hujunhong,suozhimiin |
| (Huaihe Hospital of Henan University) |
| Abstract: |
| Objective To investigate the relationship and clinical significance between the expressions of RegIV and EGFR, PI3K proteins in gastric adenocarcinoma. Methods S-P Immunohistochemistry was used to detect the expression of RegIV and EGFR, PI3K proteins in pathological tissue with 63 cases with e gastric adenocarcinoma and tumor-adjacent normal esophageal tissue. Results The positive expression rates of RegIV and EGFR, PI3K proteins in 63 cases with gastric adenocarcinoma tissue 50.7% (32/63)、68.3% (43/63) 、60.3% (38/63) respectively, were significantly different from the positive expression rates of tumor-adjacent normal esophageal tissues 19.0% (12/63)、20.6% (13/63)、9.5% (6/63)respectively(P<0.05); The expression of RegIV was significantly correlated to differentiation degree (P<0.05)and the expression of PI3K protein was significantly correlated to differentiation degree, infiltrative depth, TNM stage and lymph node metastasis(P<0.05). The expression of Akt protein was significantly correlated to lymph node metastasis (P<0.05). There was positive correlation between the expression of RegIV/PI3K, RegIV/Akt and PI3K/Akt proteins in esophageal squamous cell carcinoma and the value of Spearman coefficient correlation were 0.284,0.305 and 0.423 (P<0.05)． Conclusions RegIV may play an important role in tumorigenesis and tumor progression by activating PI3K/Akt signaling pathway. |
| Key words: regenerating gene typeIV(RegIV) gastric adenocarcinoma phosphatidylinositol-3-kinase(PI3K) Akt immunohistochemistry |
