| 摘要: |
| 长链非编码RNA（long noncoding RNA，lncRNA）的保守性表现在一级结构、空间结构、转录位置、剪接模式及组织分布等方面，是目前lncRNA研究的热点和难点。不断深入的lncRNA保守性研究可应用于参考基因组相对匮乏的非模式生物lncRNA的筛选过程，并且极大地提升了非模式生物lncRNA数据库建立的完整性和准确性。借助针对开放阅读框长度、密码子分布与出现频率、功能性结构域等保守信息开发而来的lncRNA筛选工具或流程如CPC、PLAR（pipeline for lncRNA annotation from RNA-seq data）等，已成为目前非模式生物lncRNA的筛选及其参考数据库构建的新策略。本文就lncRNA的保守性及其在非模式生物lncRNA筛选中的应用作一综述，并简要介绍了一种运用其保守性的筛选方法——PLAR。 |
| 关键词: 长链非编码RNA 保守性 非模式生物 筛选 |
| DOI：10.16781/j.0258-879x.2017.10.1304 |
| 投稿时间：2017-05-30修订日期：2017-09-11 |
| 基金项目:国家自然科学基金（81470518），上海市浦江人才项目（16PJ1411000）. |
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| Conservation of long noncoding RNA and its application in screening long noncoding RNA in non-model organisms |
| JIANG Gui-xian1,LUO Xi2,ZHANG Lu-lu3,LIU Qing3,XIAO Liang4* |
(1. The Second Student Team, Student Brigade, Second Military Medical University, Shanghai 200433, China;
2. The Eighth Student Team, Student Brigade, Second Military Medical University, Shanghai 200433, China;
3. College of Animal and Technology, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China;
4. Department of Marine Biotechnology, Faculty of Naval Medicine, Second Military Medical University, Shanghai 200433, China
*Corresponding author) |
| Abstract: |
| The conservation of long noncoding RNA (lncRNA), including primary structure, spatial structure, transcriptional position, splicing mode and tissue distribution, is research focus of lncRNA research. In-depth studies of lncRNA conservation can be applied to the screening process of lncRNA in non-modal organisms, which lack adequate reference genome databases, greatly enhancing the integrity and accuracy of lncRNA databases of non-modal organisms. LncRNA screening tools or processes such as CPC and pipeline for lncRNA annotation from RNA-seq data (PLAR), which have been developed by length of open reading frame, distribution and frequency of codon, functional domain and other conservative information, are new strategies for the screening of lncRNA and construction of reference database of non-model organisms. In this review, we summarized the conservation of lncRNA and its application in screening lncRNA in non-model organisms, and briefly introduced PLAR, a new screening method using the conservation of lncRNA. |
| Key words: long noncoding RNA conservation non-model organisms screening |
